There have been several instances of hatred toward Israeli or Jewish supporters on college campuses within the past few months, the statement said.
By signing the statement, campus officials agreed not to tolerate intimidation on campus.
The American Jewish Committee published the statement, including the 300 signatures, in an advertisement in Monday's edition of The New York Times.
The statement distinguished between anti-Semitism, which is hatred of Jews, and anti-Zionism, which is opposition to an Israeli state.
The statement also expressed the AJC's desire to initiate public action against this behavior. "We were concerned about the welfare of our students and universities," said Ken Bandler, AJC public relations director.
He said his phone has been ringing off the hook with calls from supporters of the advertisement. The AJC expects support to continue as long as the presence of anti-intimidation on campuses remains.
But several chancellors and presidents declined to sign the statement when it was originally presented to them at the beginning of the academic year by James O. Freedman, former president of Dartmouth College.
Chancellors and presidents declining to sign included leaders of Massachusetts Institute of Technology, the University of Chicago, the University of Pennsylvania and Harvard University.
An MIT representative said school officials did not sign the statement because it was too narrow in its description of groups affected by intimidation.
